Line 16 is a rapid transit line serving the south-eastern suburban areas of Shanghai. The line is colored aqua on system maps. It was originally designated as Line 21 and was planned as the south part of Line 11. The line runs entirely in Pudong New Area, starting from Longyang Road, via Shanghai Wild Animal Park, Huinan Town, ending at Dishui Lake in Nanhui New City. The line is 59 km (37 mi) long and has 13 stations of which three are underground and the rest elevated. Construction begun in early 2009, and the line opened on 29 December 2013. The second phase was completed at the end of 2014, providing interchanges to Lines 2, 7, 11, 13 and 18 (Line 18 is under construction and is to be opened in 2020).
Line 16 is the only line in the Shanghai Metro planned to feature different stopping services, with an express service stopping only at Longyang Road, Luoshan Road, Xinchang, Huinan, and Dishui Lake stations. Between 30 January 2014 and 21 March 2016, due to insufficient rolling stock and overcrowding of the line, all express services were suspended.  To further increase capacity, the existing 3 car train sets were be expanded to full 6 car sets and the rush hour headway was reduced to a minimum of 4 minutes and operated with a mixture of 6 car and 3 car trains. Since 1 October 2018, express trains also stop at Lingang Avenue station at weekends and public holidays. Since 16 November 2018, express trains of weekdays stop at Lingang Avenue station.